Funded PhD Project - MIBTP Programme

Funded PhD Project – MIBTP Programme at University of Warwick

In mammals, the process of forming sperm and eggs begins in the early embryo and is only completed at puberty. During this time, germ cells undergo a rejuvenation process that includes profound epigenetic remodelling to reset developmental potential. A major unresolved question is how environmental factors such as diet, medication, and pollutants influence this process in humans, potentially altering heritable information or contributing to disease. This is especially urgent given the global rise in infertility, now affecting ~17% of the world\’s population (WHO, 2023). A system that faithfully recapitulates human germ cell development in vitro would provide a transformative platform to address this question. Yet, recreating the embryonic microenvironments that support germ cell maturation remains a major hurdle.

Project Aim

This project aims to engineer human organoid systems that support embryonic stem cell‑derived in‑vitro germ cell (IVGC) development, enabling us to dissect how signalling cues modulate this process.

Objectives

• Identify signalling cues promoting germ cell development. Supported by the Bioinformatics & Digital Health Research and Technology Platform, we will analyse single‑cell transcriptomic profiles of human embryonic tissues to identify signalling pathways active in germ cells and their supporting somatic cells.
• Screen for conditions that promote IVGC development. In collaboration with the Kantsler lab, we will test the effects of mechanical, metabolic, and signalling cues on IVGC development, combining organoid culture with microfluidics‑based screening and multiomics profiling to capture developmental progression and reveal underlying mechanisms.
• Engineer organoid systems to advance IVGC development. Building on insights from Objectives 1 and 2, we will apply CRISPR‑based strategies to modulate gene expression, directing organoid systems to more closely resemble the niche cell types that naturally support germ cell development.

Application

If you are interested in applying for the PhD MIBTP programme (to start in October 2026), please apply through the following link: Application