A 2027 Crick PhD project with Naomi Moris.
Project background and description
Gametes (sperm and egg) originate from progenitors called ‘germ cells’ that arise early in embryo development, and are crucial for the fertility of the offspring. Although they are specified around the time of gastrulation, germ cells must migrate through various tissues – including the endodermal tract – and mature towards specific fates, with unique transcriptional and epigenetic profiles. It is not well understood how this maturation process occurs, or how interactions between somatic cells and germ cell neighbours enables mature states to arise. We will use mouse and human gastruloids[1, 2] to explore how germ cells develop in vitro using gastruloids[3], with a focus on co-developmental interactions between germ cells and other somatic tissues. The candidate will examine the role of the endoderm and somatic tissues and the various signals that play a role in germ cell fate progression, using a range of characterisation techniques and perturbation experiments. The work will explore the role of signalling interactions in germ cell biology, with the potential to reveal insights into both fundamental developmental biology principles, with future implications for exploring mechanistic causes of infertility.
Candidate background
The project would suit candidates with a very strong background in developmental biology. Desirable skills include experience of stem cell culture, organoid/embryoid experience, and background in germline research.
What do you think is one of the most important unanswered questions in developmental biology, and what makes you think it is an important question to investigate?
References
Symmetry breaking, germ layer specification and axial organisation in aggregates of mouse embryonic stem cells.
An in vitro model of early anteroposterior organization during human development.
3. Cooke, C.B., Barrington, C., Baillie-Benson, P., Nichols, J. and Moris, N. (2023)
Gastruloid-derived primordial germ cell-like cells develop dynamically within integrated tissues.
Tissue and cell interactions in mammalian PGC development.
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Moris Lab | Germ cells in gastruloids employer: Crick
The Francis Crick Institute is an exceptional employer, offering a dynamic work environment that fosters collaboration and innovation in world-class research. With a clear career path in laboratory operations, employees benefit from a supportive culture that prioritises professional growth and development, alongside a balanced work schedule that enhances work-life harmony. Located in the heart of London, the institute provides unique opportunities to contribute to groundbreaking scientific advancements while enjoying the vibrant city life.
