At a Glance
- Tasks: Lead the development of next-gen gene-editing therapeutics in a hands-on lab role.
- Company: Join a pioneering biotech firm focused on precision medicine.
- Benefits: Competitive salary, health benefits, and opportunities for professional growth.
- Other info: Fast-paced environment with exciting challenges and career advancement opportunities.
- Why this job: Make a real impact in advancing innovative therapies using CRISPR technology.
- Qualifications: M.S. or Ph.D. in relevant fields with extensive molecular cloning experience.
The predicted salary is between 50000 - 70000 £ per year.
Seeking a highly motivated scientist to lead the engineering and development of next-generation programmable gene‑editing therapeutics. This is a hands‑on laboratory role focused on designing, building, and optimizing CRISPR‑based therapeutic constructs for precision medicine applications. The successful candidate will play a key role in advancing novel therapeutic candidates from concept through preclinical development.
Responsibilities
- Design, engineer, and optimize Cas‑based therapeutic constructs, including conditional and programmable protein architectures.
- Engineer proteins for enhanced specificity, activity, and control using rational design, structure‑guided engineering, and directed evolution.
- Expand the modularity of the platform by developing constructs targeting additional disease‑relevant genes.
- Design, clone, and validate guide RNA libraries for specificity and off‑target profiling assays (e.g., ONE‑seq, CHANGE‑seq, or related technologies).
- Design, execute, and analyze rapid design‑build‑test cycles to improve construct performance.
- Collaborate across molecular biology, protein engineering, computational biology, and translational research teams to advance therapeutic programs.
Required Qualifications
- M.S. or Ph.D. in Molecular Biology, Genetics, Bioengineering, Protein Engineering, or a related field.
- At least 5 years of hands‑on experience in molecular cloning using techniques such as Gibson Assembly, Golden Gate Assembly, and Gateway cloning.
- Extensive experience designing and engineering CRISPR/Cas constructs, including development of novel protein functions through directed evolution or rational engineering.
- Strong expertise in mammalian and bacterial expression vector design, construct optimization, and stable cell line generation.
- Demonstrated ability to independently execute rapid iterative construct design and optimization.
- Experience developing and utilizing fluorescent and/or luminescent reporter assay systems.
- Strong structural biology and wet‑lab expertise, with experience applying structural insights to protein engineering challenges.
- Demonstrated ownership of engineering protein function, rather than solely supporting execution of established workflows.
- Computational experience supporting protein or CRISPR engineering, including sequence analysis, structural modeling, guide RNA design, scripting, or bioinformatics.
Preferred Qualifications
- Experience with inducible or conditional protein systems.
- Familiarity with base editing, prime editing, or other next‑generation genome editing technologies.
- Experience with NGS library preparation and sequencing‑based screening workflows.
- Background in structural biology, protein biochemistry, or computational protein engineering.
- Experience working in fast‑paced biotechnology or therapeutic discovery environments.
Molecular Cloning & Protein Engineering Scientist in London employer: Barrington James Limited
Join a well-established organisation that values its employees and fosters a supportive work culture. As an HR Associate, you will have the opportunity to be a trusted point of contact, ensuring compliance with employment legislation while contributing to the development of robust people processes. With a focus on employee growth and continuous improvement, this role offers a meaningful career path in a dynamic environment.
