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- Tasks: Explore the functions of uncharacterised testis proteins using fruit flies and advanced genetic techniques.
- Company: Join a leading research institution dedicated to groundbreaking biosciences discoveries.
- Benefits: Gain hands-on experience, access to cutting-edge resources, and potential for impactful research.
- Why this job: Dive into fascinating research with real-world implications for fertility and reproductive biology.
- Qualifications: First or upper second-class degree in a relevant field; Master's preferred for lower second-class degrees.
- Other info: Opportunity to work with both fruit flies and mammalian models, enhancing your research portfolio.
The predicted salary is between 36000 - 60000 £ per year.
Normal animal reproduction depends on the appropriate production of gametes – sperm and egg, and on their successful interaction (fertilisation). Sperm are extremely specialised cells; their success in fertilisation requires them to be free living, motile, and able to fuse with the egg to activate embryonic development. About 20% of the protein coding genes in the genome are exclusively or almost exclusively expressed in testes. The function of some of these genes is known, for example the transition proteins and protamines that remodel the sperm DNA. However, the biological function of the majority of testis-specifically expressed proteins has never been determined. They are members of the Unknome – the proteome of unknown function.
In this project you will systematically analyse the functions of the testis Unknome proteins, exploring both lineage specific proteins and proteins more widely conserved in evolution. You will capitalise on the extensive genetic resources available for fruit flies (D. melanogaster), particularly the large collections of mutants including those derived from gene disruption projects, including an ongoing CRISPR screen that aims to mutate every gene in D. melanogaster. You will assess the phenotypes of flies mutant for testis unknome genes, and thus determine their role in fertility.
Drosophila spermatogenesis is particularly amenable to cytological analysis. Testes from young adult flies contain all stages of spermatogenesis, from stem cells through to mature sperm. Defects in many critical cell biology processes, such as stem cell maintenance, cell division, mitochondrial morphogenesis, axoneme elongation and nuclear shaping are readily identified by phase contrast microscopy, with the opportunity for more detailed analysis via imaging of GFP-tagged marker proteins or immuno-fluorescence. Fertility assays will reveal any defects in the fly strains that are able to produce morphologically normal sperm. You will be able to select one or more (groups of) genes that you find particularly interesting for more in depth analysis. Some of the testis unknome proteins are conserved to mammals. You will have the opportunity to investigate how these genes are important for mammalian sperm function, to complement the fly work. The localization of homologous gene products in mouse testis and mouse or pig spermatozoa will be studied with immunocytochemistry using custom made polyclonal antibodies. If proteins are surface localized the effect of antibodies on sperm motility, mitochondrial activity, the acrosome reaction or sperm induced egg fusion will be assayed.
How to apply: You can apply online - consideration is automatic on applying for a PhD in Biosciences. Please use our online application service at Cardiff University. Please specify that you are applying for this particular project, the supervisor and source of funding. Information on the application process can be found here.
Academic criteria: Applicants should have obtained, or be about to obtain, a first or upper second-class UK honours degree, or the equivalent qualification gained outside the UK. Applicants with a lower second class will only be considered if they also have a Master’s degree. Please check the entry requirements of the home institution for each project of interest before completing an application. Academic qualifications are considered alongside significant relevant non-academic experience.
English requirements: If English is not your first language you will need to meet the English language requirements of the university that will host your PhD by the start of the programme. Please refer to the relevant university website for further information.
Exploring the testis unknome. Functional analysis of uncharacterised testis-specifically expres[...] employer: Cardiff University
Contact Detail:
Cardiff University Recruiting Team
StudySmarter Expert Advice 🤫
We think this is how you could land Exploring the testis unknome. Functional analysis of uncharacterised testis-specifically expres[...]
✨Tip Number 1
Familiarise yourself with the latest research on testis-specific proteins and the Unknome. This will not only help you understand the project better but also allow you to engage in meaningful discussions during interviews, showcasing your genuine interest and knowledge.
✨Tip Number 2
Connect with current or past researchers in the field of Drosophila genetics. Networking can provide you with insights into the lab culture and expectations, which can be invaluable when preparing for interviews or discussions about your fit for the role.
✨Tip Number 3
Prepare to discuss your hands-on experience with techniques relevant to the project, such as CRISPR, microscopy, or immunocytochemistry. Being able to articulate your practical skills will demonstrate your readiness for the research challenges ahead.
✨Tip Number 4
Show enthusiasm for interdisciplinary approaches. Since the project involves both fruit flies and mammalian models, expressing your interest in how findings from one can inform the other will highlight your broader understanding of biological research.
We think you need these skills to ace Exploring the testis unknome. Functional analysis of uncharacterised testis-specifically expres[...]
Some tips for your application 🫡
Understand the Project: Read through the project description carefully. Make sure you grasp the key concepts, such as the role of testis-specific proteins and the methodologies involved, like CRISPR screening and immunocytochemistry.
Tailor Your CV: Highlight relevant academic qualifications and any research experience related to genetics, molecular biology, or cytology. Emphasise skills that align with the project, such as microscopy techniques or experience with Drosophila.
Craft a Strong Personal Statement: In your personal statement, express your passion for the subject and explain why this specific project excites you. Discuss any relevant experiences and how they have prepared you for this research opportunity.
Follow Application Instructions: When applying, ensure you specify the project title, supervisor, and funding source as instructed. Use the online application service provided and double-check all details before submission.
How to prepare for a job interview at Cardiff University
✨Know Your Science
Make sure you have a solid understanding of the biological concepts related to gamete production and spermatogenesis. Brush up on the roles of testis-specific proteins and be ready to discuss how they might relate to your research interests.
✨Familiarise Yourself with Drosophila Research
Since the project involves D. melanogaster, it’s crucial to understand its genetics and the significance of using fruit flies in biological research. Be prepared to talk about any relevant experience you have with genetic resources or mutant collections.
✨Prepare Questions
Interviews are a two-way street. Prepare insightful questions about the project, the lab environment, and potential collaborations. This shows your genuine interest and helps you assess if it's the right fit for you.
✨Highlight Relevant Experience
If you have any experience with microscopy, immunocytochemistry, or fertility assays, make sure to highlight this during the interview. Relating your skills to the specific techniques mentioned in the project will demonstrate your suitability.
